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![Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/MDMX-Antibody-Western-Blot-NB100-556-img0008.jpg "Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDMX-Antibody-Western-Blot-NB100-556-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot NB100-556-4) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (lot NB100-556-3) and another rabbit anti-HdmX/MDM4 antibody. For blotting immunoprecipitated HdmX/MDM4, NB100-556 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDMX-Antibody-Immunoprecipitation-NB100-556-img0009.jpg)
![Western Blot: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.](http://images.novusbio.com/fullsize/MDMX-Antibody-2D10F4-Western-Blot-NBP1-28862-img0014.jpg "Western Blot: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.](http://images.novusbio.com/fullsize/MDMX-Antibody-2D10F4-Immunocytochemistry-Immunofluorescence-NBP1-28862-img0011.jpg)
![Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human Tonsil tissues using MDM4 mouse mAb](http://images.novusbio.com/fullsize/MDMX-Antibody-2D10F4-Immunohistochemistry-Paraffin-NBP1-28862-img0013.jpg)
![Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.](http://images.novusbio.com/fullsize/MDMX-Antibody-2D10F4-Immunohistochemistry-Paraffin-NBP1-28862-img0012.jpg)
![Western Blot: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDMX.](http://images.novusbio.com/fullsize/MDMX-Antibody-OTI4G5-Western-Blot-NBP2-45779-img0005.jpg "Western Blot: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDMX.")
![Immunocytochemistry/Immunofluorescence: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MDM4.](http://images.novusbio.com/fullsize/MDMX-Antibody-OTI4G5-Immunocytochemistry-Immunofluorescence-NBP2-45779-img0001.jpg)
![Immunohistochemistry: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MDMX-Antibody-OTI4G5-Immunohistochemistry-NBP2-45779-img0004.jpg)
![Immunohistochemistry: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MDMX-Antibody-OTI4G5-Immunohistochemistry-NBP2-45779-img0002.jpg)
![Immunohistochemistry: MDMX Antibody (OTI4G5) [NBP2-45779] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MDMX-Antibody-OTI4G5-Immunohistochemistry-NBP2-45779-img0003.jpg)
![Western Blot: MDMX Antibody [NBP2-58791] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MDMX-Antibody-Western-Blot-NBP2-58791-img0002.jpg "Western Blot: MDMX Antibody [NBP2-58791] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: MDMX Antibody [NBP2-58791] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MDMX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58791-img0001.jpg)
![Immunohistochemistry-Paraffin: MDMX Antibody [NBP1-87733] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MDMX-Antibody-Immunohistochemistry-Paraffin-NBP1-87733-img0003.jpg "Immunohistochemistry-Paraffin: MDMX Antibody [NBP1-87733] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.")
![Western Blot: MDMX Overexpression Lysate (Adult Normal) [NBL1-12971] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDMX.](http://images.novusbio.com/fullsize/MDMX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12971-img0002.jpg "Western Blot: MDMX Overexpression Lysate (Adult Normal) [NBL1-12971] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDMX.")
![Western Blot: MDMX Antibody [NBP2-97044] - Anti-MDMX rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 68 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/MDMX-Antibody-Western-Blot-NBP2-97044-img0002.jpg "Western Blot: MDMX Antibody [NBP2-97044] - Anti-MDMX rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 68 kDa (We are unsure as to the identity of these extra bands).")