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MDL-1/CLEC5A Antibody (283834) [Unconjugated]

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Human peripheral blood granulocytes were stained with (A) MouseAnti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL‑1/CLEC5A Monoclonal Antibody (Catalog # MAB2384, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Human peripheral blood granulocytes were stained with (A) MouseAnti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL‑1/CLEC5A Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
283834
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2384U-100UG
Alexa Fluor 405
FAB2384V-100UG
Alexa Fluor 488
FAB2384G-100UG
Alexa Fluor 532
FAB2384X
Alexa Fluor 594
FAB2384T-100UG
Alexa Fluor 647
FAB2384R-100UG
Alexa Fluor 700
FAB2384N-100UG
Alexa Fluor 750
FAB2384S-100UG
Allophycocyanin
FAB2384A
Allophycocyanin/Cy7
FAB2384APCCY7
Biotin
FAB2384B
CoraFluor 1
FAB2384CL1
DyLight 350
FAB2384D
DyLight 405
FAB2384E
DyLight 488
FAB2384K
DyLight 550
FAB2384L
DyLight 594
FAB2384M
DyLight 650
FAB2384W
DyLight 680
FAB2384Y
DyLight 755
FAB2384Z
FITC
FAB2384F
HRP
FAB2384H
Janelia Fluor 525
FAB2384JF525
Janelia Fluor 549
FAB2384I
Janelia Fluor 646
FAB2384J
Janelia Fluor 669
FAB2384JF669
PE/Atto594
FAB2384PEATT594
PE/Cy5.5
FAB2384PECY55
PE/Cy7
FAB2384PECY7
Phycoerythrin
FAB2384P
mFluor Violet 450 SE
FAB2384MFV450
mFluor Violet 500 SE
FAB2384MFV500
mFluor Violet 610 SE
FAB2384MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2384G-100UG
Carrier Free
FAB2384A
Carrier Free
FAB2384P
Carrier Free
MAB2384-SP
Carrier Free
FAB2384V-100UG
Carrier Free
FAB2384U-100UG
Carrier Free
FAB2384T-100UG
Carrier Free
FAB2384S-100UG
Carrier Free
FAB2384R-100UG
Carrier Free
FAB2384N-100UG
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MDL-1/CLEC5A Antibody (283834) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25
Specificity
Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC5A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2384 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDL-1/CLEC5A Antibody (283834) [Unconjugated]

  • CLEC5A
  • CLECSF5
  • CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5
  • C-type lectin domain family 5, member A
  • C-type lectin superfamily member 5
  • MDL1
  • MDL-1
  • MDL-1MGC138304
  • Myeloid DAP12-associating lectin 1
  • myeloid DAP12-associating lectin-1

Background

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDL-1/CLEC5A Antibody (MAB2384)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol CLEC5A
Uniprot