![Western Blot: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 cell lysates using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Western-Blot-NBP3-08389-img0002.jpg "Western Blot: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 cell lysates using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | rMCM7/1468 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07277). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary) (Uniprot: P33993) |
| Localization | Nuclear |
| Marker | Proliferation Marker |
| Specificity | The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCM7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 88 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MCM7 Antibody (NBP3-08389)Find related products by research area.
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| Gene Symbol | MCM7 |
![Immunohistochemistry-Paraffin: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Immunohistochemistry-Paraffin-NBP3-08389-img0001.jpg "Immunohistochemistry-Paraffin: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).")
![Protein Array: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).](http://images.novusbio.com/fullsize/MCM7-Antibody-rMCM7-1468-Protein-Array-NBP3-08389-img0003.jpg "Protein Array: MCM7 Antibody (rMCM7/1468) [NBP3-08389] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Recombinant Mouse Monoclonal Antibody (rMCM7/1468).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")