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MCM3 Antibody (2H3)

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Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in human ovarian cancer.
Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in human ovarian cancer.
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).
Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in Y-79 (Cat # L042V1).
ELISA: MCM3 Antibody (2H3) [H00004172-M04] - Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MCM3 Antibody (2H3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
Specificity
MCM3 - MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), (2H3)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCM3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM3 Antibody (2H3)

  • cervical cancer proto-oncogene 5
  • DNA polymerase alpha holoenzyme-associated protein P1
  • DNA replication factor MCM3
  • DNA replication licensing factor MCM3
  • EC 3.6.4.12
  • HCC5
  • hRlf beta subunit
  • MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)
  • MCM3 minichromosome maintenance deficient 3
  • MGC1157
  • minichromosome maintenance complex component 3
  • minichromosome maintenance deficient (S. cerevisiae) 3
  • minichromosome maintenance deficient 3
  • P1.h
  • p102
  • P1-MCM3
  • replication licensing factor, beta subunit
  • RLF subunit beta
  • RLFB

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00004172-M04
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Bioinformatics

Gene Symbol MCM3
Entrez
Uniprot