We wish to measure MASP-2 levels in human plasma. Are stored plasma (or serum) at -20 centigrade stabile for this measurement if only frozen once. Should we measure in duplicate or triplicate? Is plasma better than serum as I have both stored for the MASP-2 test? What would be the cost of a kit and how many samples would it measure? I assume there is no special equipment other than ELISA like stuff? What is the shelf life as I have holiday next month? Is the a list of equipment we need to run the kits so I can check it with our lab technicians.

Name: MASP2 Antibody (narsoplimab)
Brand: Novus Biologicals
SKU: NBP3-28061
Price: 279 USD

Question

Accepted Answer

Once frozen samples should be fine for measurement. Generally, all experiments should be performed at least in triplicate so you can perform statistical analysis. MASP-2 should be detectable in both serum and plasma. Serum might yield cleaner signals since it is depleted of blood cells and clotting factors, but the depletion could, theoretically remove some MSAP-2 as well. Either type of sample should be suitable though. We currently have no MASP2 ELISA kits available. A list of our MASP2 antibodies can be found at this link if you would like to make your own. We guarantee all of our antibodies for 6 months from the receipt date.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA, Flow, Func
Clone	narsoplimab
Clonality	Monoclonal
Host	Human
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-28061AF350

Alexa Fluor 405 NBP3-28061AF405

Alexa Fluor 488 NBP3-28061AF488

Alexa Fluor 532 NBP3-28061AF532

Alexa Fluor 594 NBP3-28061AF594

Alexa Fluor 647 NBP3-28061AF647

Alexa Fluor 700 NBP3-28061AF700

Alexa Fluor 750 NBP3-28061AF750

Allophycocyanin NBP3-28061APC

Biotin NBP3-28061B

CoraFluor 1 NBP3-28061CL1

DyLight 350 NBP3-28061UV

DyLight 405 NBP3-28061V

DyLight 488 NBP3-28061G

DyLight 550 NBP3-28061R

DyLight 594 NBP3-28061DL594

DyLight 650 NBP3-28061C

DyLight 680 NBP3-28061FR

DyLight 755 NBP3-28061IR

FITC NBP3-28061F

HRP NBP3-28061H

Janelia Fluor 525 NBP3-28061JF525

Janelia Fluor 549 NBP3-28061JF549

Janelia Fluor 646 NBP3-28061JF646

Janelia Fluor 669 NBP3-28061JF669

PE NBP3-28061PE

PerCP NBP3-28061PCP

mFluor Violet 450 SE NBP3-28061MFV450

mFluor Violet 500 SE NBP3-28061MFV500

mFluor Violet 610 SE NBP3-28061MFV610

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28061AF350
Alexa Fluor 405	NBP3-28061AF405
Alexa Fluor 488	NBP3-28061AF488
Alexa Fluor 532	NBP3-28061AF532
Alexa Fluor 594	NBP3-28061AF594
Alexa Fluor 647	NBP3-28061AF647
Alexa Fluor 700	NBP3-28061AF700
Alexa Fluor 750	NBP3-28061AF750
Allophycocyanin	NBP3-28061APC
Biotin	NBP3-28061B
CoraFluor 1	NBP3-28061CL1
DyLight 350	NBP3-28061UV
DyLight 405	NBP3-28061V
DyLight 488	NBP3-28061G
DyLight 550	NBP3-28061R
DyLight 594	NBP3-28061DL594
DyLight 650	NBP3-28061C
DyLight 680	NBP3-28061FR
DyLight 755	NBP3-28061IR
FITC	NBP3-28061F
HRP	NBP3-28061H
Janelia Fluor 525	NBP3-28061JF525
Janelia Fluor 549	NBP3-28061JF549
Janelia Fluor 646	NBP3-28061JF646
Janelia Fluor 669	NBP3-28061JF669
PE	NBP3-28061PE
PerCP	NBP3-28061PCP
mFluor Violet 450 SE	NBP3-28061MFV450
mFluor Violet 500 SE	NBP3-28061MFV500
mFluor Violet 610 SE	NBP3-28061MFV610

Description	Expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa. Also known as 'narsoplimab'. Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
Additional Information	Recombinant Monoclonal Antibody
Immunogen	MASP2
Isotype	IgG4
Clonality	Monoclonal
Host	Human
Gene	MASP2
Purity	Protein A purified
Endotoxin Note	< 0.001EU/ug,determined by LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

MASP2 Antibody (narsoplimab)

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Storage	Store at 4C.
Buffer	Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
Preservative	No Preservative
Concentration	LYOPH
Purity	Protein A purified
Reconstitution Instructions	Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Gene Symbol	MASP2
Uniprot	O00187(Human)