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MAP4 Antibody

Images

 
Western Blot: MAP4 Antibody [NBP1-89483] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Immunocytochemistry/ Immunofluorescence: MAP4 Antibody [NBP1-89483] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human kidney.
Independent Antibodies: Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human kidney, skeletal muscle, soft tissues and testis using Anti-MAP4 antibody NBP1-89483 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human skeletal muscle.
Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human soft tissues.
Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human testis.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

MAP4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGT
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAP4 Protein (NBP1-89483PEP)
Publications
Read Publication using NBP1-89483.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAP4 Antibody

  • DKFZp779A1753
  • MAP-4
  • MGC8617
  • microtubule-associated protein 4

Background

MAP4 is encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP4 Antibody (NBP1-89483)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP4 Antibody (NBP1-89483) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on MAP4

There are no specific blogs for MAP4, but you can read our latest blog posts.
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Contact Information

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Bioinformatics

Gene Symbol MAP4