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MAP4 Antibody

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Independent Antibodies: Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 ...read more
Immunohistochemistry: MAP4 Antibody [NB100-74616] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-MAP4 used at a dilution of 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

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MAP4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1102 and 1152 of human microtubule-associated protein 4 using the numbering given in entry NP_002366.2 (GeneID 4134).
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAP4 Antibody

  • DKFZp779A1753
  • MAP-4
  • MGC8617
  • microtubule-associated protein 4

Background

The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP4
Entrez
Uniprot