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Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free

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Western Blot: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate Isomerase (Right ...read more
Immunocytochemistry/ Immunofluorescence: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mannose Phosphate Isomerase.
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Human pancreas tissue using anti-Mannose Phosphate Isomerase mouse monoclonal ...read more
Flow Cytometry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were ...read more
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Mannose Phosphate Isomerase ...read more
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Mannose Phosphate Isomerase mouse ...read more
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Mannose Phosphate Isomerase mouse ...read more
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Mannose Phosphate Isomerase mouse ...read more
Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free [NBP2-72582] - Staining of paraffin-embedded Human prostate tissue using anti-Mannose Phosphate Isomerase mouse monoclonal ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MPI(NP_002426) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MPI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Mannose Phosphate Isomerase Antibody (OTI1C7) - Azide and BSA Free

  • CDG1B
  • EC 5.3.1
  • EC 5.3.1.8
  • FLJ39201
  • mannose phosphate isomerase
  • mannose-6- phosphate isomerase
  • mannose-6-phosphate isomerase
  • Phosphohexomutase
  • phosphomannose isomerase 1
  • Phosphomannose isomerase
  • PMI
  • PMI1phosphohexomutase

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MPI