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M-Ras/R-Ras3 Antibody

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Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for M-Ras/R-Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3606-SP
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M-Ras/R-Ras3 Antibody Summary

Immunogen
E. coli-derived recombinant human M-Ras
Met1-Leu208 (Lys181Glu)
Accession # O14807
Specificity
Detects human and rat M-Ras/R-Ras3 in Western blots. Does not detect recombinant human R-Ras or TC21 (R-Ras2).
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MRAS
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publication using
AF3606 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-Ras/R-Ras3 Antibody

  • FLJ42964
  • MRas
  • M-Ras
  • muscle and microspikes RAS
  • muscle RAS oncogene homolog
  • ras-related protein M-Ras
  • Ras-related protein R-Ras3
  • R-RAS3
  • RRAS3M-RAs

Background

Muscle Ras (M-Ras), also known as R-Ras3, is a small GTP-binding protein of the Ras superfamily of GTPases. M-Ras comprises an R-Ras subfamily with fellow members R-Ras and TC21 (R-Ras2). While highly homologous elsewhere, M-Ras differs at its carboxyl terminus from both R-Ras and TC21, suggesting this GTPase may interact with novel signaling effectors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-Ras/R-Ras3 Antibody (AF3606)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol MRAS
Uniprot