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LSM1 Antibody (OTI5C6)

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Western Blot: LSM1 Antibody (5C6) [NBP2-00918] Analysis of extracts (35ug) from 9 different cell lines by using anti-LSM1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LSM1 Antibody (5C6) [NBP2-00918] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1.
Immunohistochemistry-Paraffin: LSM1 Antibody (5C6) [NBP2-00918] - Staining of paraffin-embedded Human Kidney tissue using anti-LSM1 mouse monoclonal antibody.
Flow Cytometry: LSM1 Antibody (5C6) [NBP2-00918] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by ...read more
Western Blot: LSM1 Antibody (5C6) [NBP2-00918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: LSM1 Antibody (5C6) [NBP2-00918] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LSM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LSM1 Antibody (5C6) [NBP2-00918] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LSM1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LSM1 Antibody (5C6) [NBP2-00918] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LSM1 mouse monoclonal antibody.
Flow Cytometry: LSM1 Antibody (5C6) [NBP2-00918] - Analysis of Hela cells, using anti-LSM1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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LSM1 Antibody (OTI5C6) Summary

Immunogen
Full length human recombinant protein of human LSM1 (NP_055277) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LSM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LSM1 Antibody (OTI5C6)

  • Cancer-associated Sm-like
  • CASMYJL124C
  • LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
  • Small nuclear ribonuclear CaSm
  • U6 snRNA-associated Sm-like protein LSm1

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LSM1
Entrez