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LRRC32/GARP Antibody [Unconjugated]

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Western blot shows lysates of mouse spleen non-B cells. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse LRRC32/GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRC32/GARP at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse spleen non-B cells. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse LRRC32/GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) followed by ...read more
LRRC32/GARP was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Sheep Anti-Mouse LRRC32/ GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) at 10 µg/mL for 3 hours at room ...read more
LRRC32/GARP was detected in immersion fixed paraffin-embedded sections of Mouse Spleen using Sheep Anti-Mouse LRRC32/GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) at 0.3 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6229-SP
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LRRC32/GARP Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP
Ile18-Asn628
Accession # NP_001106850
Specificity
Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LRRC32 is observed and less than 5% cross-reactivity with rhLRRC3, rhLRRC4, and rhLRRC4B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LRRC32
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF6229 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRC32/GARP Antibody [Unconjugated]

  • D11S833E
  • D11S833Eleucine-rich repeat-containing protein 32
  • GARP
  • GARPGarpin
  • Garpin
  • Glycoprotein A repetitions predominantgarpin
  • leucine rich repeat containing 32
  • LRRC32

Background

Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2-4). Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6-8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
  1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
  2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
  3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
  4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
  5. Macaulay, I.C. et al. (2007) Blood 109:3260.
  6. Wang, R. et al. (2008) PloS ONE 3:e2705.
  7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
  8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
  9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
  10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
  11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LRRC32/GARP
Antibody [Unconjugate
AF6229
LRRC32/GARP Antibody [Unconjugated]
Species: Mu
Applications: WB, IHC, ICC/IF

Publications for LRRC32/GARP Antibody (AF6229)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol LRRC32
Uniprot