![Immunohistochemistry: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31992) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31992_rabbit-lmw-ptp-acp1-mab-psh04-57-298202414333658.jpg "Immunohistochemistry: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31992) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | PSH04-57 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human LMW-PTP/ACP1 aa 1-158 / 158. (Uniprot: P24666) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ACP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ACP1 |
![Western Blot: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Western blot analysis of LMW-PTP/ACP1 on different lysates with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute 33 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31992_rabbit-lmw-ptp-acp1-mab-psh04-57-3920248453523.jpg "Western Blot: LMW-PTP/ACP1 Antibody (PSH04-57) [NBP3-31992] - Western blot analysis of LMW-PTP/ACP1 on different lysates with Rabbit anti-LMW-PTP/ACP1 antibody (NBP3-31992) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute 33 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
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