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Lipin 1 Antibody [Biotin]

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Lipin 1 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human Lipin 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3885) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Lipin 1 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human Lipin 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3885) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF3885
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3885
Carrier Free
AF3885
Carrier Free
AF3885-SP
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Lipin 1 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693
Specificity
Detects human Lipin 1 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LPIN1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipin 1 Antibody [Biotin]

  • DKFZp781P1796
  • EC 3.1.3.18
  • EC 3.1.3.4
  • EC 5.4.2.7
  • KIAA0188phosphatidate phosphatase LPIN1
  • Lipin 1
  • lipin-1
  • LPIN1
  • PAP1

Background

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha , respectively. Lipin 1 beta is cytoplasmic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LPIN1
Uniprot