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LEPRE1 Antibody

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Western Blot: LEPRE1 Antibody [H00064175-B01P] - Analysis of LEPRE1 expression in human placenta.
Western Blot: LEPRE1 Antibody [H00064175-B01P] - Analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 polyclonal antibody. Lane 1: LEPRE1 transfected lysate(42.9 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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LEPRE1 Antibody Summary

Immunogen
LEPRE1 (AAH15309, 1 a.a. - 390 a.a.) full-length human protein. MLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKLLNGSQRVVMDGVISDHECQELQRLTNVAATSGDGYRGQTSPHTPNEKFYGVTVFKALKLGQEGKVPLQSAHLYYNVTEKVRRIMESYFRLDTPLYFSYSHLVCRTAIEEVQAERKDDSHPVHVDNCILNAETLVCVKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEMDLSQEQPLDAQQGPPEPAQESLSGSESKPKDEL
Specificity
LEPRE1 - leucine proline-enriched proteoglycan (leprecan) 1,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
P3H1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and tissue lysate for Western Blot.
Publications
Read Publications using
H00064175-B01P in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LEPRE1 Antibody

  • EC 1.14.11.7
  • GROS1OI8
  • Growth suppressor 1
  • leprecan
  • Leprecan-1
  • Leucine- and proline-enriched proteoglycan 1
  • leucine proline-enriched proteoglycan (leprecan) 1
  • MGC117314
  • P3H1LEPRECAN
  • prolyl 3-hydroxylase 1

Background

This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LEPRE1 Antibody (H00064175-B01P)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Human
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using H00064175-B01P Applications Species
Besio R, Garibaldi N, Leoni L et al. Cellular stress due to impairment of collagen prolyl hydroxylation complex is rescued by the chaperone 4-phenylbutyrate Dis Model Mech. 2019-06-20 [PMID: 31171565]
Khan M, Zukowska J, Jung J et al. Dextromethorphan inhibits collagen transport in the endoplasmic reticulum eliciting an anti-fibrotic response inex-vivoandin vitromodels of pulmonary fibrosis bioRxiv 2023-04-20 (ICC/IF, Human) ICC/IF Human
Huang Y, Mei L, Lv W et al. Targeted exome sequencing identifies novel compound heterozygous mutations in LEPRE1 in a fetus with osteogenesis imperfecta type VIII. Clin Chim Acta 2016-11-15 [PMID: 27864101]
Cabral WA, Perdivara I, Weis M et al. Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta. PLoS Genet. 2014-06-26 [PMID: 24968150]
Takagi M, Ishii T, Barnes AM et al. A Novel Mutation in LEPRE1 That Eliminates Only the KDEL ER- Retrieval Sequence Causes Non-Lethal Osteogenesis Imperfecta. PLoS One. 2012-05-15 [PMID: 22615817]
Amor IM, Rauch F, Gruenwald K et al. Severe osteogenesis imperfecta caused by a small in-frame deletion in CRTAP. Am J Med Genet A. 2011-09-30 [PMID: 21964860]
Pyott SM, Schwarze U, Christiansen HE et al. Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. Hum Mol Genet. 2011-02-13 [PMID: 21282188]
Baldridge D, Lennington J, Weis M et al. Generalized connective tissue disease in Crtap-/- mouse. PLoS One 5(5). 2010-05-11 [PMID: 20485499]
Chang W, Barnes AM, Cabral WA et al. Prolyl 3-hydroxylase 1 and CRTAP are mutually stabilizing in the endoplasmic reticulum collagen prolyl 3-hydroxylation complex. Hum Mol Genet;19(2):223-34. 2010-01-15 [PMID: 19846465]
van Dijk FS, Nesbitt IM, Zwikstra EH et al. PPIB mutations cause severe osteogenesis imperfecta. Am J Hum Genet 2009-10-01 [PMID: 19781681]
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LEPRE1 Antibody (H00064175-B01P) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol P3H1
Entrez
Uniprot