HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (MAB002, open histogram), followed by ...read more
A172 cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated ...read more
U-118-MG cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more
Chinese hamster ovary cell line CHO-derived recombinant human LDLR Ala22-Arg788 Accession # P01130
Specificity
Detects human LDLR in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDLR, recombinant human LRP-5, or rmLRP-6 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LDLR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for LDLR Antibody (472413) [Unconjugated]
FH
FHC
LDL R
LDL receptor
LDLCQ2
LDLR
low density lipoprotein receptor
low-density lipoprotein receptor class A domain-containing protein 3
low-density lipoprotein receptor
Background
The Low Density Lipoprotein Receptor (LDLR) is the founding member of the LDLR family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDLR is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDLR is 51% aa identical to the extracellular region of human VLDLR, and 79% aa identical to the extracellular region of mouse LDLR. LDLR is constitutively expressed and binds ApoB of LDL and ApoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).
Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
Yamamoto, T. et al. (1984) Cell 39:27.
Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
Varret, M. et al. (2008) Clin Genet. 73:1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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