Home » Laminin alpha 3/Laminin-5 » Laminin alpha 3/Laminin-5 Antibodies » Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]
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Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]

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Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room ...read more
Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, ICC/IF
Clone
P3H9-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2144-SP
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Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated] Summary

Immunogen
Human keratinocytes
Specificity
Detects human Laminin alpha 3/Laminin‑5 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMA3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot
Publications
Read Publications using
MAB2144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]

  • BM600 150kD subunit
  • BM600
  • E170
  • Epiligrin 170 kDa subunit
  • epiligrin alpha 3 subunit
  • Epiligrin
  • JEB2A
  • JEB2B
  • kalinin 165kD subunit
  • Kalinin subunit alpha
  • LAM3, alpha-3 subunit
  • LAMA3
  • laminin A3
  • Laminin alpha 3
  • laminin subunit alpha-3
  • laminin-5 alpha 3 chain
  • Laminin-5 subunit alpha
  • Laminin5
  • Laminin-5
  • Laminin-6 subunit alpha
  • Laminin-7 subunit alpha
  • LAMNA
  • LOCS
  • nicein 150kD subunit
  • Nicein subunit alpha

Background

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.
  3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2144
Laminin alpha 3/Laminin-5 Antibody (P3H9-2) [Unconjugated]
Species: Hu
Applications: WB, IHC, IP, B/N, ICC/IF

Publications for Laminin alpha 3/Laminin-5 Antibody (MAB2144)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, Immunohistochemistry, Neutralization.


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ICC/IF
(1)
Immunohistochemistry
(1)
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(1)
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Showing Publications 1 - 5 of 5.
Publications using MAB2144 Applications Species
Gao, H;Huang, X;Cai, Z;Cai, B;Wang, K;Li, J;Kuang, J;Wang, B;Zhai, Z;Ming, J;Cao, S;Qin, Y;Pei, D; Generation of musculoskeletal cells from human urine epithelium-derived presomitic mesoderm cells Cell & bioscience 2024-07-15 [PMID: 39010176] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
J Zwicklhube, T Kocher, B Liemberger, S Hainzl, J Bischof, D Strunk, AM Raninger, I Gratz, V Wally, C Guttmann-G, JP Hofbauer, JW Bauer, U Koller A Novel Fluorescence-Based Screen of Gene Editing Molecules for Junctional Epidermolysis Bullosa International Journal of Molecular Sciences, 2023-03-08;24(6):. 3/8/2023 [PMID: 36982270]
SD Newby, T Masi, CD Griffin, WJ King, A Chipman, S Stephenson, DE Anderson, AS Biris, SE Bourdo, M Dhar Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis Int J Nanomedicine, 2020-04-15;15(0):2501-2513. 2020-04-15 [PMID: 32368037] (ICC/IF, Human) ICC/IF Human
CJ Pendegrass, Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, Stanmore, UK, HT Lancashire, C Fontaine, G Chan, P Hosseini et al. Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro European Cells and Materials 4/19/2015 [PMID: 25890596]
Houghton AM, Quintero PA, Perkins DL, Kobayashi DK, Kelley DG, Marconcini LA, Mecham RP, Senior RM, Shapiro SD Elastin fragments drive disease progression in a murine model of emphysema. J. Clin. Invest., 2006-02-09;116(3):753-9. 2006-02-09 [PMID: 16470245] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol LAMA3