![Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Western-Blot-NBP2-71097-img0002.jpg "Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | OTI2A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | L1CAM |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 140 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for L1CAM Antibody (NBP2-71097)Find related products by research area.
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| Gene Symbol | L1CAM |
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71097-img0005.jpg "Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0013.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.")
![Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71097-img0004.jpg "Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Western-Blot-NBP2-71097-img0001.jpg "Western Blot: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0006.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0007.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0008.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0009.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0010.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0011.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.")
![Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71097-img0012.jpg "Immunohistochemistry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.")
![Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71097-img0003.jpg "Flow Cytometry: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Immunoprecipitation of L1CAM by using a monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Azide-and-BSA-Free-Immunoprecipitation-NBP2-71097-img0014.jpg "Immunoprecipitation: L1CAM Antibody (OTI2A6) - Azide and BSA Free [NBP2-71097] - Immunoprecipitation of L1CAM by using a monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")