![Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of KRT9 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/KRT9-Antibody-SD201-09-Western-Blot-NBP2-67810-img0002.jpg "Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of KRT9 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | SD201-09 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein wiehin Human KRT9 aa 234-422 / 623. (SwissProt: P35527 Human; SwissProt: Q6RHW0 Mouse; SwissProt: Q8CIS9 Rat) |
| Localization | Extracellular exosome, membrane, nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT9 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for KRT9 Antibody (NBP2-67810)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT9 |
![Immunocytochemistry/Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of MCF-7 cells labeling KRT9 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody KRT9 (red) at 1/200 dilution for overnight at 4 . Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/KRT9-Antibody-SD201-09-Immunocytochemistry-Immunofluorescence-NBP2-67810-img0003.jpg "Immunocytochemistry/Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of MCF-7 cells labeling KRT9 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody KRT9 (red) at 1/200 dilution for overnight at 4 . Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 (washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0005.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 (washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of mouse skin tissue using anti-Cytokeratin 9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0001.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of mouse skin tissue using anti-Cytokeratin 9 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/KRT9-Antibody-SD201-09-Immunohistochemistry-Paraffin-NBP2-67810-img0004.jpg "Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![N/A alpha-Defensin 1 [Biotin]](https://images.novusbio.com/images2/DATA_alphaDefensin_1_DY819805_ELISA_2534.jpg)
![Simple Western Cytokeratin 18 Antibody (810811) [Unconjugated]](https://images.novusbio.com/images2/16390.jpg)
![Western Blot Cytokeratin 18 Antibody (810811) [Unconjugated]](https://images.novusbio.com/images2/Cytokeratin_18_MAB7619_Western_Blot_12720.jpg)
![Immunocytochemistry Cytokeratin 18 Antibody (810811) [Unconjugated]](https://images.novusbio.com/images2/Cytokeratin_18_MAB7619_Immunocytochemistry_12734.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")