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KLF11 Antibody (10D8)

Images

 
Western Blot: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of KLF11 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on NIH/3T3 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: KLF11 Antibody (10D8) [H00008462-M03] - Analysis of monoclonal antibody to KLF11 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
Chromatin Immunoprecipitation: KLF11 Antibody (10D8) [H00008462-M03] - 5 ug KLF11 antibody on human adipocytes - data from a customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, ChIP
Clone
10D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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KLF11 Antibody (10D8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
KLF11 (NP_003588, 404 a.a. ~ 512 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TYFKSSHLKAHLRTHTGEKPFNCSWDGCDKKFARSDELSRHRRTHTGEKKFVCPVCDRRFMRSDHLTKHARRHMTTKKIPGWQAEVGKLNRIASAESPGSPLVSMPASA
Specificity
KLF11 - Kruppel-like factor 11 (10D8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KLF11
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, ELISA, ChIP and IHC-P. Use in Immunoprecipitation reported in scientific literature (PMID: 23408111).
Reviewed Applications
Read 1 Review rated 4
using
H00008462-M03 in the following applications:

Publications
Read Publications using
H00008462-M03 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KLF11 Antibody (10D8)

  • FKLFFKLF1
  • Krueppel-like factor 11
  • Kruppel-like factor 11
  • MODY7TGFB-inducible early growth response protein 2
  • TIEG-2
  • TIEG2TGFB inducible early growth response 2
  • Tieg3
  • Transforming growth factor-beta-inducible early growth response protein 2

Background

Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF11 Antibody (H00008462-M03)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Chemotaxis, ICC/IF, IF/IHC, IHC-P, IP, WB.


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Chemotaxis
(1)
ICC/IF
(1)
IF/IHC
(2)
IHC-P
(1)
IP
(1)
WB
(3)
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Human
(3)
Mouse
(3)
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Showing Publications 1 - 8 of 8.
Publications using H00008462-M03 Applications Species
Lin L, Pfender K, Ditsch N et al. KLF11 is an independent negative prognostic factor for breast cancer from a cohort study and induces proliferation and inhibits apoptosis in vitro Breast cancer (Tokyo, Japan) 2023-05-18 [PMID: 37199905] (IHC-P, Human) IHC-P Human
Ambrogio L, Maria O, Luigi V et al. Survivin, Sonic hedgehog, Kruppel-like factors, and p53 pathway in serous ovarian cancer: an immunohistochemical study. Hum Pathol. 2022-06-28 [PMID: 35777700]
De Lorenzo SB, Vrieze AM, Johnson RA et al. KLF11 deficiency enhances chemokine generation and fibrosis in murine unilateral ureteral obstruction PloS one [PMID: 35413089] (IF/IHC, Mouse) IF/IHC Mouse
Tang X, Liu K, Hamblin MH et al. Genetic Deletion of Kruppel-Like Factor 11 Aggravates Ischemic Brain Injury. Mol. Neurobiol. 2017-04-29 [PMID: 28456933] (WB, Mouse) WB Mouse
Mathison A, Escande C, Calvo E et al. Phenotypic Characterization of Mice Carrying Homozygous Deletion of KLF11, a Gene in Which Mutations Cause Human Neonatal and MODY VII Diabetes. Endocrinology 2015-10-01 [PMID: 26248217]
Loft A, Forss I, Siersbaek MS et al. Browning of human adipocytes requires KLF11 and reprogramming of PPARgamma superenhancers. Genes Dev 2015-01-01 [PMID: 25504365] (Chemotaxis, WB, Human) Chemotaxis, WB Human
Yin KJ, Fan Y, Hamblin M et al. KLF11 mediates PPARgamma cerebrovascular protection in ischaemic stroke. Brain 2013-02-12 [PMID: 23408111] (WB, IP, Mouse) WB, IP Mouse
Lin L The role of Tumor-associated Macrophages (TAMs) and Transcription Factor KLF11 in breast cancer (BC) and KLF11-mediated Transcriptional Regulation of BC-associated TAMs Thesis 2022-01-01 (ICC/IF, IF/IHC, Human) ICC/IF, IF/IHC Human

Review for KLF11 Antibody (H00008462-M03) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00008462-M03:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation KLF11 H00008462-M03
Enlarge
4
reviewed by:
Anne Loft
ChIP Human 12/02/2013
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHuman adipocytes
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLF11 Antibody (H00008462-M03) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anne Loft
12/02/2013
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol KLF11
Entrez
Uniprot