Novus Biologicals products are now on bio-techne.com

KIR3DL1 Antibody (177407) [Alexa Fluor® 750]

Images

 
There are currently no images for KIR3DL1 Antibody (FAB12251S).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
177407
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

KIR3DL1 Antibody (177407) [Alexa Fluor® 750] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human KIR3DL1
Accession # P43629
Specificity
Detects human KIR3DL1 on transfected BaF/3 cells. When tested against a panel of transfectants, no cross-reactivity with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL2, or 3DS1 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
KIR3DL1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR3DL1 Antibody (177407) [Alexa Fluor® 750]

  • AMB11
  • CD158 antigen-like family member E
  • CD158e antigen
  • CD158E
  • CD158e1
  • CD158e1/2
  • CD158e2
  • cl-11
  • cl-2
  • HLA-BW4-specific inhibitory NK cell receptor
  • killer cell immunoglobulin-like receptor 3DL1
  • killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3
  • killer Ig receptor
  • KIR antigen 3DL1
  • KIR3DL1
  • KIR3DL1/S1
  • KIR3DS1
  • MGC119726
  • MGC119728
  • MGC126589
  • MGC126591
  • MHC class I NK cell receptor
  • Natural killer-associated transcript 3
  • Nkat3
  • NKAT-3
  • NKAT3KIR
  • NKB1
  • NKB1B
  • NKB1CD158E1
  • NK-receptor
  • p70 killer cell inhibitory receptor
  • p70 natural killer cell receptor clones CL-2/CL-11
  • p70 NK receptor CL-2/CL-11

Background

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1‑3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40‑48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Uhrberg, M. et al. (1997) Immunity 7:753.
  4. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
  5. Thananchai, H. et al. (2007) J. Immunol. 178:33.
  6. Martin, M.P. et al. (2007) Nat. Genet. 39:733.
  7. Pando, M.J. et al. (2003) J. Immunol. 171:6640.
  8. Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
  9. Wilson, E.B. et al. (2007) Immunogenetics 59:641.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-50419
Species: Hu
Applications: CyTOF-ready, Flow, IP
MAB1058
Species: Hu
Applications: Block, CyTOF-ready, Flow
MAB2014
Species: Hu
Applications: CyTOF-reported, Flow
MAB1844
Species: Hu
Applications: CyTOF-reported, Flow
MAB8887
Species: Hu
Applications: Flow
NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
MAB2238
Species: Hu
Applications: AgAct, CyTOF-reported, Flow
MAB1059
Species: Hu
Applications: CyTOF-ready, Flow
H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
3015-KR
Species: Hu
Applications: BA
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP2-36776
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-82874
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP3-03813
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-12900
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, MiAr, Simple Western, WB
AF2408
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB

Publications for KIR3DL1 Antibody (FAB12251S) (0)

There are no publications for KIR3DL1 Antibody (FAB12251S).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for KIR3DL1 Antibody (FAB12251S) (0)

There are no reviews for KIR3DL1 Antibody (FAB12251S). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for KIR3DL1 Antibody (FAB12251S) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KIR3DL1 Antibody (177407) [Alexa Fluor® 750] and receive a gift card or discount.

Bioinformatics

Gene Symbol KIR3DL1
Uniprot