Ki67/MKI67 Antibody

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP2-19012	Applications	Species
Deng Z, Shen D, Yu M et al. Pectolinarigenin inhibits bladder urothelial carcinoma cell proliferation by regulating DNA damage/autophagy pathways Cell Death Discovery 2023-07-01 [PMID: 37393350]
Chen S, Wang Y, Xiong Y et al. Wild-type IDH1 inhibits the tumor growth through degrading HIF-alpha in renal cell ijbs.com 2021-01-01 [PMID: 33867843] (WB, Human)	WB	Human
Song Chen, Qiang Zhou, Zicheng Guo, Yejinpeng Wang, Lu Wang, Xuefeng Liu, Mengxin Lu, Lingao Ju, Yu Xiao, Xinghuan Wang Inhibition of MELK produces potential anti‐tumour effects in bladder cancer by inducing G1/S cell cycle arrest via the ATM/CHK2/p53 pathway Journal of Cellular and Molecular Medicine 2019-12-10 [PMID: 31821699]
Chen L, Peng T, Luo Y et al. ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis Front Oncol 2019-10-09 [PMID: 31649873] (ICC/IF, Human)	ICC/IF	Human
Cheng S, Qian K, Wang Y, et al. PPAR-gamma inhibition regulates the cell cycle, proliferation and motility of bladder cancer cells J. Cell. Mol. Med. 2019-05-01 [PMID: 30912275] (ICC/IF, Human)	ICC/IF	Human
Hu Q, Wang G et al. Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways. Biomed Res Int 2017-11-18 [PMID: 29147649] (ICC/IF, Human)	ICC/IF	Human
Rui Cao, Gang Wang, Kaiyu Qian, Liang Chen, Guofeng Qian, Conghua Xie, Han C. Dan, Wei Jiang, Min Wu, Chin-Lee Wu, Yu Xiao, Xinghuan Wang Silencing of HJURP induces dysregulation of cell cycle and ROS metabolism in bladder cancer cells via PPAR gamma -SIRT1 feedback loop Journal of Cancer 2017-01-01 [PMID: 28819432]
Chen L, Wang G, Luo Y et al. Downregulation of LAPTM5 suppresses cell proliferation and viability inducing cell cycle arrest at G0/G1 phase of bladder cancer cells. Int. J. Oncol. 2017-01-01 [PMID: 27922670]
Qian K, Wang G, Cao R et al. Capsaicin Suppresses Cell Proliferation, Induces Cell Cycle Arrest and ROS Production in Bladder Cancer Cells through FOXO3a-Mediated Pathways. Molecules 2016-10-21 [PMID: 27775662]
Zhou L, Xu M, Yang Y et al. Activation of beta-Catenin Signaling in CD133-Positive Dermal Papilla Cells Drives Postnatal Hair Growth PLoS ONE 2016-07-30 [PMID: 27472062] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhou L, Yang K, Carpenter A et al. CD133-positive dermal papilla-derived Wnt ligands regulate postnatal hair growth. Biochem. J. 2016-10-01 [PMID: 27462123] (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 11 Publications. Collapse Publications.

Array NBP2-19012

• Ki67/MKI67 Antibodies
• Ki67/MKI67 Proteins
• Ki67/MKI67 ELISA