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Ki67/MKI67 Antibody [Unconjugated]

 (8 Publications)
 (1 Review)
 
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Ki67/MKI67 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Ki67/MKI67 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 5 µg/mL for 3 hours at room ...read more
Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 1 µg/mL ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Ki67 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at approximately 325 ...read more
Genetic Strategies: Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Sheep Anti-Human Ki67/MKI67 ...read more
MKi67 mRNA (red) and MKi67 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, IHC, Dual ISH-IHC, ICC/IF, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF7617-SP
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Ki67/MKI67 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Ki67/MKI67
Asn3120-Ile3256
Accession # P46013
Specificity
Detects human Ki67/MKI67 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Ki67/MKI67 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MKI67
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 20 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF7617 in the following applications:
Publications
Read Publications using
AF7617 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ki67/MKI67 Antibody [Unconjugated]

  • antigen identified by monoclonal Ki-67
  • Antigen Ki67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-
  • MIB-1
  • MKI67
  • PPP1R105
  • Proliferation Marker Protein Ki-67
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

MKI67 (also Ki67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki67 shares 46% aa sequence identity with the mouse ortholog to Ki67.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki67/MKI67 Antibody (AF7617)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(3)
IHC-P
(1)
Western Blot
(1)
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Human
(5)
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Showing Publications 1 - 8 of 8.
Publications using AF7617 Applications Species
TE Hoffman, C Yang, V Nangia, CR Ill, SL Spencer Multiple cancer types rapidly escape from multiple MAPK inhibitors to generate mutagenesis-prone subpopulations bioRxiv : the preprint server for biology, 2023-03-21;0(0):. 2023-03-21 [PMID: 36993538] (IHC, Human) IHC Human
M Denechaud, S Geurs, T Comptdaer, S Bégard, A Garcia-Núñ, LA Pechereau, T Bouillet, Y Vermeiren, PP De Deyn, R Perbet, V Deramecour, CA Maurage, M Vanderhaeg, S Vanuytven, B Lefebvre, E Bogaert, N Déglon, T Voet, M Colin, L Buée, B Dermaut, MC Galas Tau promotes oxidative stress-associated cycling neurons in S phase as a pro-survival mechanism: possible implication for Alzheimer's disease Progress in neurobiology, 2022-12-06;0(0):102386. 2022-12-06 [PMID: 36481386] (IHC, Human) IHC Human
Madalena Cipriano, Katharina Schlünder, Christopher Probst, Kirstin Linke, Martin Weiss, Mona Julia Fischer et al. Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs Communications Biology 1/13/2022 [PMID: 35027657]
Zhipeng Ge, Haibo Liu, Tao Ji, Qiaoling Liu, Lulu Zhang, Pengchong Zhu et al. Long non-coding RNA 00960 promoted the aggressiveness of lung adenocarcinoma via the miR-124a/SphK1 axis Bioengineered 1/9/2022 [PMID: 34738865]
Y Lin, B Li, X Yang, Q Cai, W Liu, M Tian, H Luo, W Yin, Y Song, Y Shi, R He Fibroblastic FAP promotes intrahepatic cholangiocarcinoma growth via MDSCs recruitment Neoplasia, 2019-11-20;21(12):1133-1142. 2019-11-20 [PMID: 31759251] (IHC, Human) IHC Human
J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma Oncol Lett, 2018-04-13;15(6):9009-9016. 2018-04-13 [PMID: 29844818] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
Zhe Yuan, Guobin Kang, Lance Daharsh, Wenjin Fan, Qingsheng Li SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model Emerging Microbes & Infections 12/1/2018 [PMID: 29615603]
J Kim, H Okamoto, Z Huang, G Anguiano, S Chen, Q Liu, K Cavino, Y Xin, E Na, R Hamid, J Lee, B Zambrowicz, R Unger, AJ Murphy, Y Xu, GD Yancopoulo, WH Li, J Gromada Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic ? Cell Hyperplasia in Mice Cell Metab., 2017-06-06;25(6):1348-1361.e8. 2017-06-06 [PMID: 28591637] (Human) Human

Review for Ki67/MKI67 Antibody (AF7617) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF7617:
Filter by Applications
ICC
(1)
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Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Ki67/MKI67 AF7617
Enlarge 		5
reviewed by:
Natalie Erdmann
 ICC Human 08/24/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedMultiple myeloma cells line LP-1 cytospins
SpeciesHuman
LotCGSE011507C

FAQs for Ki67/MKI67 Antibody (AF7617). (Showing 1 - of FAQs).

    Secondary Antibodies

    		 

    Isotype Controls

    Additional Ki67/MKI67 Products

    Blogs on Ki67/MKI67.

    Check out the latest blog posts on Ki67/MKI67.
    The relationship between Ki67 and HIF-1 in cancer
    Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.
    Ki67 - an established marker for labelling proliferating cells
    Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.
    Ki67 - A Crucial Cellular Proliferation Marker
    The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.
    The Ki67 Antibody in Cell Marker Studies
    The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is ...  Read full blog post.
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    Immunocytochemistry Ki67/MKI67 AF7617
    Enlarge
    		 Natalie Erdmann
    08/24/2015
    Application: ICC
    Species: Human
    read this review

    Bioinformatics

    Gene Symbol MKI67
    Uniprot