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Pig anti-Human Kappa Light Chain Secondary Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, WB
Clonality
Polyclonal
Host
Pig
Conjugate
Unconjugated
Concentration
LYOPH

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Pig anti-Human Kappa Light Chain Secondary Antibody Summary

Description
The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Immunogen
A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma.
Clonality
Polyclonal
Host
Pig
Gene
IGKC
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot
Application Notes
Indirect immunofluorescence.

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Alternate Names for Pig anti-Human Kappa Light Chain Secondary Antibody

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGKC