Reactivity | HuSpecies Glossary |
Applications | WB, DB, ELISA, ICC/IF, WB |
Clonality | Polyclonal |
Host | Pig |
Conjugate | Unconjugated |
Concentration | LYOPH |
Description | The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
Immunogen | A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. |
Clonality | Polyclonal |
Host | Pig |
Gene | IGKC |
Purity | Ion exchange chromatography |
Dilutions |
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Application Notes | Indirect immunofluorescence. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from PBS (pH 7.2) |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | Ion exchange chromatography |
Reconstitution Instructions | Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. |
Research Areas for Kappa Light Chain Antibody (NBP2-69238)Find related products by research area.
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Gene Symbol | IGKC |