Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 800] Summary
Description |
Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 800 (Ex = 777 nm; Em = 794 nm). After reconstituted, prepare fresh working dilution daily
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. |
Immunogen |
Purified Human kappa light chain |
Specificity |
Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
IGKC |
Purity |
Affinity purified |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:20 - 1:2000
- Immunomicroscopy 1:20 - 1:2000
|
Application Notes |
This conjugate is suitable for immunomicroscopy and flow cytometry. |
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins
Packaging, Storage & Formulations
Storage |
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage. |
Buffer |
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free |
Preservative |
0.05% Sodium Azide |
Concentration |
LYOPH |
Purity |
Affinity purified |
Reconstitution Instructions |
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
Notes
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 800]
Background
Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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