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Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 800]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 800
Concentration
LYOPH

Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 800] Summary

Description
Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 800 (Ex = 777 nm; Em = 794 nm). After reconstituted, prepare fresh working dilution daily

Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Immunogen
Purified Human kappa light chain
Specificity
Based on IEP, this antibody reacts with kappa and light chains on all human immunoglobulins
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGKC
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:2000
  • Immunomicroscopy 1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, heavy chains on all human immunoglobulins, and lambda light chains on all human immunoglobulins

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody [DyLight 800]

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGKC