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ISG15/UCRP Antibody

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Western blot shows lysates of DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human ISG15/UCRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4845) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of DLD-1 clone 2C2 human colon adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and CCD-1070Sk human foreskin fibroblast cell line. PVDF membrane was probed with 1 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4845-SP
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ISG15/UCRP Antibody Summary

Immunogen
E. coli-derived recombinant human ISG15
Gly2-Gly157
Accession # ABW03539
Specificity
Detects endogenous human ISG15 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ISG15
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF4845 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ISG15/UCRP Antibody

  • G1P2
  • G1P2interferon-induced 17-kDa/15-kDa protein
  • IFI15
  • IFI15hUCRP
  • interferon, alpha-inducible protein (clone IFI-15K)
  • Interferon-induced 15 kDa protein
  • Interferon-induced 17 kDa protein
  • interferon-stimulated protein, 15 kDa
  • IP17
  • ISG15 ubiquitin-like modifier
  • ISG15
  • Ubiquitin cross-reactive protein
  • ubiquitin-like protein ISG15
  • UCRP
  • UCRPIP17

Background

ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation, instead, it is part of the anti‑virus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 15‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ISG15/UCRP Antibody (AF4845)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Capture.


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Bioinformatics

Gene Symbol ISG15
Uniprot