Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG ...read more
Intelectin‑1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin‑1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was ...read more
E. coli-derived recombinant mouse Intelectin‑1/Omentin Ala20-Ser298 Accession # O88310
Specificity
Detects mouse Intelectin‑1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ITLN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Intelectin-1/Omentin Antibody (746420)
Endothelial lectin HL-1
FLJ20022
Galactofuranose-binding lectin
hIntL
HL-1
intelectin 1 (galactofuranose binding)
Intelectin1
Intelectin-1
INTLHL1
ITLN1
Itlna
ITLNITLN-1
LFR
LFRIntestinal lactoferrin receptor
Omentin
Omentin-1
Background
Intelectin (endothelial lectin; also ITLN-1 and omentin) is a 34 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed in intestinal and airway epithelium and appears to participate in insulin signaling and microbe recognition. Intelectin-1 is upregulated in asthma and contributes to allergic airway inflammation. The mouse Intelectin-1 preproprecursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature segment (aa 20-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. Soluble Intelectin-1 can be released from omental adipose tissue and malignant pleural mesothelioma. One fibrinogen-related domain exists in the mature molecule (aa 32-251). Human Intelectin-1 exists as a disulfide-linked glycosylated homotrimer; mouse is unglycosylated and a monomer. Mature mouse Intelectin-1 is 82% aa identical to human Intelectin-1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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