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integrin beta 4 binding protein Antibody (A6A3-R)

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Western Blot: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody ...read more
Immunohistochemistry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-integrin beta 4 binding protein antibody ...read more
Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunocytochemistry analysis of HepG2 cells labeling integrin beta 4 binding protein with Mouse anti-integrin ...read more
Flow Cytometry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Flow cytometric analysis of HepG2 cells labeling integrin beta 4 binding protein. Cells were fixed and permeabilized. Then stained with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
A6A3-R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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integrin beta 4 binding protein Antibody (A6A3-R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within human integrin beta 4 binding protein aa 1-200. (Uniprot: P56537)
Localization
Cytoplasm, Nucleus, nucleolus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF6
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for integrin beta 4 binding protein Antibody (A6A3-R)

  • B(2)GCN homolog
  • b(2)gcn
  • B4 integrin interactor
  • CAB
  • EIF3Ap27(BBP)
  • eIF-6
  • eukaryotic translation initiation factor 3A
  • eukaryotic translation initiation factor 6
  • ITGB4BPintegrin beta 4 binding protein
  • p27 beta-4 integrin-binding protein
  • p27BBP

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF6