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Importin-9 Antibody

Images

 
Genetic Strategies: Knockout Validated: Importin-9 Antibody [NB100-56489] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. ...read more
Western Blot: Importin-9 Antibody [NB100-56489] - Analysis of Importin-9 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 5 ug/ml.
Immunohistochemistry-Paraffin: Importin-9 Antibody [NB100-56489] - Analysis of human brain, cortex using this antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Pm, RMSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

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Importin-9 Antibody Summary

Immunogen
A portion of amino acids 950-1000 of human Importin-9 was used as the immunogen.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_b and CRA_c), (isoforms 1 and 2 and Importin-9a and Importin-9b),
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IPO9
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Knockout Validated 2 ug/ml
  • Western Blot 0.5-2 ug/ml

Reactivity Notes

Chicken (82%), Rat, Bovine (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Importin-9 Antibody

  • DKFZp761M1547
  • FLJ10402
  • Imp9IMP9
  • importin 9
  • importin-9
  • KIAA1192
  • Ran-binding protein 9
  • ranBP9

Background

Official Gene Symbol: IPO9 Gene ID: 55705 (Human) Gene Map Locus: 1q32.1 (Human) Importin 9 is a novel member of highly conserved Karyopherin Super family. Karyopherins are transport receptors consisting of an N-terminal RanGTP-binding motif and mediate the transport of protein and RNA across the nucleus and cytoplasm. These proteins interact directly with nuclear pore complex and shuttle continuously between the nucleus and cytoplasm. Importin-9 is a RAN-GTP binding protein and mediates the nuclear import of individual core histones and TBP. Reports suggest Importin-9 also interacts with subunits of PP2A (protein phosphatase 2A) and aids in nuclear import.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional Importin-9 Products

Array NB100-56489

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Bioinformatics

Gene Symbol IPO9
Entrez
Uniprot