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Rat IL-3 ELISA Kit (Colorimetric)

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ELISA: Rat IL-3 ELISA Kit (Colorimetric) [NBP3-06776] - Samples were spiked with high concentrations of Rat IL-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat IL-3 ELISA Kit (Colorimetric) [NBP3-06776] - Samples were spiked with high concentrations of Rat IL-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat IL-3 ELISA Kit (Colorimetric) [NBP3-06776] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Rat IL-3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat IL-3 in samples. No significant cross-reactivity or interference between Rat IL-3 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.45
Intra-Assay
6.06
Spike Recovery
92 - 108 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL3

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat IL-3 ELISA Kit (Colorimetric)

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Manual - Rat IL-3 ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol IL3