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IL-20R alpha Antibody

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Western Blot: IL-20 R alpha Antibody [NB100-56409] - Analysis of IL-20 R alpha Antibody in A375 cell lysate in the (A) absence and (B) presence of blocking peptide using this antibody at 2 ug/ml.
Western Blot: IL-20 R alpha Antibody [NB100-56409] - Analysis of IL-20 R alpha Antibody in A375 cell lysate in the (A) absence and (B) presence of blocking peptide using this antibody at 2 ug/ml.
Flow Cytometry: IL-20 R alpha Antibody [NB100-56409] - Cell-surface flow cytometry analysis of IL-20 R alpha Antibodyin A375 cells using this antibody at 5 ug/10^6 cells. The shaded histogram represents cells only, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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IL-20R alpha Antibody Summary

Immunogen
Amino acids 159-175 of human IL-20 receptor alpha protein were used as the immunogen.
Specificity
Both the expect ~70 kD band and an unknown ~90 kD band are seen in A375 cell lysate and are blocked by the immunizing peptide. The ~90 kD band alone is seen in A431 cell lysate, and it is also blocked by the immunizing peptide. This antibody was made against a portion of the extracellular domain of IL-20RA. Permeabilization of the cells during testing may affect antibody binding.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL20RA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2-5 ug/10^6 cells
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human and chimp, and 82% homologous in mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IL-20R alpha Antibody

  • class II cytokine receptor ZCYTOR7
  • CRF2-8
  • Cytokine receptor class-II member 8
  • Cytokine receptor family 2 member 8
  • FLJ40993
  • IL-20 R alpha
  • IL20R alpha
  • IL-20R alpha
  • IL-20R1
  • IL-20R1IL-20 receptor subunit alpha
  • IL20RA
  • IL-20Ra
  • IL-20R-alpha
  • interleukin 20 receptor, alpha
  • interleukin-20 receptor I
  • interleukin-20 receptor subunit alpha
  • zcytor7

Background

Official Gene Symbol: IL20RA Gene ID: 53832 (Human) Gene Map Locus: 6q22.33-q23.1 (Human) IL20RA, is a subunit of IL20R, a member of Cytokine Class II receptor super family. It consists of a central transmembrane domain and functionally induces IL20 and IL26 signaling in epithelial cells and keratinocytes. It associates with IL-20RB and forms a functional receptor complex for IL20, IL19, and IL24. It also associates with IL-10RB and forms a unique heterodimeric functional receptor complex for IL-26. Upon ligand binding, it induces signaling through activation of STAT3 and STAT1. IL20RA is specifically expressed in skin suggesting a prominent role in growth and proliferation of keratinocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-20R alpha
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NB100-56409
IL-20R alpha Antibody
Species: Hu, Mu
Applications: WB, Flow
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Bioinformatics

Gene Symbol IL20RA
Entrez