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Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

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ELISA: Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP1-94192]
ELISA: Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP1-94192]

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
39.1 - 2,500 pg/mL
Sensitivity
23.0 pg/mL

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Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric) Summary

Description
The pig IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of pig IL-18
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a pig IL-18 positive serum. There was no crossreactivity detected
Standard Curve Range
39.1 - 2,500 pg/mL
Sensitivity
23.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.1%
Intra-Assay
CV% < 6.8%
Spike Recovery
The overall mean recovery was 110%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL18

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to pig IL-18
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-pig IL-18 monoclonal antibody
  5. Pig IL-18 Standard lyophilized, 5 ng/ml upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells. In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1. The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Manual - Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol IL18