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Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Supernatant, Cell Lysates, Plasma (EDTA, Heparin), Serum
Standard Curve Range
15.6 to 1000 pg/mL
Sensitivity
<1 pg/mL

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Human IL-18/IL-1F4 ELISA Kit (Colorimetric) Summary

Description
IL18 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human IL18.
Standard Curve Range
15.6 to 1000 pg/mL
Sensitivity
<1 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL18

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using KA0561.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 96-well plate precoated with anti- human IL-18 antibody
  2. ABC diluent buffer
  3. Antibody diluent buffer
  4. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  5. Biotinylated anti- human IL-18 antibody, dilution 1:100
  6. Lyophilized recombinant human IL-18 standard
  7. Sample diluent buffer
  8. TMB color developing agent
  9. TMB stop solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

The protein encoded by the IL18 gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for IL-18/IL-1F4 ELISA Kit (KA0561)(8)

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(1)
WB
(1)
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Showing Publications 1 - 8 of 8.
Publications using KA0561 Applications Species
Yen-Fu C, Ao-Ho H, Yao-Fan F et al. Diagnostic Evaluation Using Salivary Gland Ultrasonography in Primary Sjogren‘s Syndrome. J Clin Med. 2023-03-22 [PMID: 36983428]
Chi K, Geng X, Liu C Et Al. LncRNA-HOTAIR promotes endothelial cell pyroptosis by regulating the miR-22/NLRP3 axis in hyperuricaemia Journal of cellular and molecular medicine 2021-07-22 [PMID: 34296520] (ELISA) ELISA
Peng G, Ching O, Abdalla A et al. IFN-? and IL-18 in conditioned media of parasite-infected host and IL-21-silenced colorectal cancer cells. Exp Ther Med. 2020-11-27 [PMID: 33335566]
Liu T, Yang P, Chen H et al. Global analysis of differential gene expression related to long-term sperm storage in oviduct of Chinese Soft-Shelled Turtle Pelodiscus sinensis. Sci Rep 2016-09-15 [PMID: 27628424] (WB) WB
Guo W, Ye P, Yu H et al. CD24 activates the NLRP3 inflammasome through c-Src kinase activity in a model of the lining epithelium of inflamed periodontal tissues. Immunity, Inflammation and Disease. 2014-01-01 [PMID: 25866631]
Reddy P, Teshima T, Kukuruga M et al. Interleukin-18 regulates acute graft-versus-host disease by enhancing Fas-mediated donor T cell apoptosis. J Exp Med. 2001-11-19 [PMID: 11714750]
Mallat Z, Corbaz A, Scoazec A et al. Expression of interleukin-18 in human atherosclerotic plaques and relation to plaque instability. Circulation. 2001-10-02 [PMID: 11581135]
Okamura H, Tsutsi H, Komatsu T et al. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature. 1995-11-02 [PMID: 7477296]

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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
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  IL18 (Human) ELISA Kit
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Bioinformatics

Gene Symbol IL18