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IL-17F Antibody (197301) [Unconjugated]

 (3 Publications)
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Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with ...read more
IL-17F was detected in immersion fixed human T helper 17 cells using Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13351) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
197301
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC13351U-100UG
Alexa Fluor 405
IC13351V-100UG
Alexa Fluor 488
IC13351G-100UG
Alexa Fluor 532
FAB13351X
Alexa Fluor 594
IC13351T-100UG
Alexa Fluor 647
IC13351R-100UG
Alexa Fluor 700
IC13351N-100UG
Alexa Fluor 750
IC13351S-100UG
Allophycocyanin
FAB13351A
Biotin
FAB13351B
CoraFluor 1
FAB13351CL1
DyLight 350
FAB13351D
DyLight 405
FAB13351E
DyLight 488
FAB13351K
DyLight 550
FAB13351L
DyLight 594
FAB13351M
DyLight 650
FAB13351W
DyLight 680
FAB13351Y
DyLight 755
FAB13351Z
HRP
FAB13351H
Janelia Fluor 525
FAB13351JF525
Janelia Fluor 549
FAB13351I
Janelia Fluor 646
FAB13351J
Janelia Fluor 669
FAB13351JF669
PerCP
FAB13351C
mFluor Violet 450 SE
FAB13351MFV450
mFluor Violet 500 SE
FAB13351MFV500
mFluor Violet 610 SE
FAB13351MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB13351-SP
Carrier Free
IC13351V-100UG
Carrier Free
IC13351U-100UG
Carrier Free
IC13351T-100UG
Carrier Free
IC13351S-100UG
Carrier Free
IC13351R-100UG
Carrier Free
IC13351N-100UG
Carrier Free
IC13351G-100UG
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IL-17F Antibody (197301) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-17F
Arg31-Gln163
Accession # Q96PD4
Specificity
Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL17F
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB13351 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody (197301) [Unconjugated]

  • Cytokine ML-1
  • IL17F
  • IL-17F
  • IL-17Finterleukin-17F
  • interleukin 17F

Background

Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-17F Antibody
(197301) [Unconjugate
MAB13351
IL-17F Antibody (197301) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF

Publications for IL-17F Antibody (MAB13351)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, Neutralization.


Filter By Application
IHC
(1)
IHC-P
(1)
Neutralization
(1)
All Applications
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Human
(3)
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Bioinformatics

Gene Symbol IL17F
Uniprot