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Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric)

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ELISA: Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric) [NBP3-06744] - Samples were spiked with high concentrations of Rat IL-12/IL-35 p35 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric) [NBP3-06744] - Samples were spiked with high concentrations of Rat IL-12/IL-35 p35 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric) [NBP3-06744] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

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Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat IL-12 p70/IL-12A in samples. No significant cross-reactivity or interference between Rat IL-12 p70/IL-12A and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.4
Intra-Assay
5.78
Spike Recovery
87 - 109 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL12A

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric)

  • CLMF p35
  • CLMF
  • cytotoxic lymphocyte maturation factor 1, p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12, subunit p35
  • IL-12/IL-35 p35
  • IL-12A
  • IL35 subunit
  • IL35p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • NF cell stimulatory factor chain 1
  • NFSK
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Rat IL-12/IL-35 p35 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol IL12A