![Western Blot: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)](http://images.novusbio.com/fullsize/IgM-Antibody-IM260-Azide-and-BSA-Free-Western-Blot-NBP2-34649-img0001.jpg "Western Blot: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, CyTOF-ready |
| Clone | IM260 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34253). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant heavy chain of human IgM |
| Localization | Cytoplasm, Cell Surface and Secreted |
| Marker | B-Cell Marker |
| Specificity | Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGHM |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP2-34649 | Applications | Species |
|---|---|---|
| Suzuki O Regulation of cell adhesion to galectins by glycosylation: A new concept in lymphoma cell adhesion Advances in Cancer Biology - Metastasis 2021-11-01 (AdAct, Human) | AdAct | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Mariko Momohara |
IHC-P | Human | 07/13/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for IgM Antibody (NBP2-34649)Find related products by research area.
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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp... Read full blog post. |
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Read full blog post. |
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How To Identify B Cell Subsets Using Flow Cytometry By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHM |
![Immunohistochemistry-Paraffin: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Human lymph node. IgM antibody at 1:200 at RT for 1 hr. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IgM-Antibody-IM260-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34649-img0003.jpg "Immunohistochemistry-Paraffin: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Human lymph node. IgM antibody at 1:200 at RT for 1 hr. IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM Antibody (IM260)](http://images.novusbio.com/fullsize/IgM-Antibody-IM260-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34649-img0002.jpg "Immunohistochemistry-Paraffin: IgM Antibody (IM260) - Azide and BSA Free [NBP2-34649] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM Antibody (IM260)")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
