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IgM Antibody (1.9/3.2)

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Product Details

Summary
Reactivity Rt, EqSpecies Glossary
Applications ELISA, Flow
Clone
1.9/3.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

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IgM Antibody (1.9/3.2) Summary

Description
This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).
Immunogen
Horse immunoglobulins
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGHM
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
Application Notes
Use in ELISA reported in scientific literature (PMID:35042674)
Publications
Read Publications using
NBP2-61099 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34440885). .

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Notes

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Alternate Names for IgM Antibody (1.9/3.2)

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Background

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Antibody (NBP2-61099)(5)

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Secondary Antibodies

 

Isotype Controls

Additional IgM Products

Research Areas for IgM Antibody (NBP2-61099)

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Blogs on IgM.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHM