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Porcine IgG ELISA Kit (Colorimetric)

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ELISA: Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Porcine IgG ELISA Kit (Colorimetric) [NBP2-60065] - Standard Curve Reference

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernates and tissue homogenates
Standard Curve Range
0.586 ug/mL - 150 ug/mL
Sensitivity
0.467 ug/mL

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Porcine IgG ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of pig IgG. No significant cross-reactivity or interference between pig IgG and analogues was observed.
Standard Curve Range
0.586 ug/mL - 150 ug/mL
Sensitivity
0.467 ug/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGHG1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-60065.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Sample Diluent
  6. Standard
  7. Stop Solution
  8. TMB Substrate
  9. Wash Buffer (25 x concentrate)

Alternate Names for Porcine IgG ELISA Kit (Colorimetric)

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with pig IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for pig IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of pig IgG in samples. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for IgG ELISA Kit (NBP2-60065)(1)

We have publications tested in 1 application: ELISA.


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Contact Information

Product PDFs

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  Manual - Porcine IgG ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol IGHG1