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Rat IgG ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Biological Samples, Plasma, Serum
Standard Curve Range
25 to 800 ng/mL
Sensitivity
2.894

Order Details

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Rat IgG ELISA Kit (Colorimetric) Summary

Description
IgG (Rat) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of IgG in rat biological samples.
Standard Curve Range
25 to 800 ng/mL
Sensitivity
2.894
Assay Type
Two-site enzyme-linked immunoassay
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGHG1

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using KA1952.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Anti-Rat IgG ELISA Micro Plate: Twelve removable eight (8) well strips in well holderframe. Each well is coated with affinity purified anti-Rat IgG.
  2. Chromogen-Substrate Solution: One vial containing 3,3',5,5'-tetramethylbenzidine(TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  3. Diluent Concentrate (Running buffer): One bottle containing 5X concentrated diluentrunning buffer
  4. Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti- Rat IgGantibody conjugated with horseradish peroxidase in a stabilizing buffer.
  5. Rat IgG Calibrator: One vial containing a lyophilized Rat IgG calibrator.
  6. Stop Solution: One vial containing 0.3 M sulfuric acid.WARNING: Avoid contact with skin.
  7. Wash Solution Concentrate: One bottle containing 20X concentrated wash solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat IgG ELISA Kit (Colorimetric)

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IgG ELISA Kit (KA1952) (0)

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Research Areas for IgG ELISA Kit (KA1952)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1