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Donkey anti-Rabbit IgG Secondary Antibody [NL557]

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Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL557

Order Details

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Catalog# & Conjugate Size Price
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Donkey anti-Rabbit IgG Secondary Antibody [NL557] Summary

Additional Information
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Rabbit IgG
Specificity
Rabbit IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry
Publications
Read Publications using
NL004 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL004)(40)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Snail, Transgenic Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry.


Filter By Application
Flow Cytometry
(1)
ICC
(8)
IHC
(9)
IHC-Fr
(3)
IHC-P
(2)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(9)
Rat
(3)
Snail
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NL004 Applications Species
Hussain, SS;Libby, EF;Lever, JEP;Tipper, JL;Phillips, SE;Mazur, M;Li, Q;Campos-Gómez, J;Harrod, KS;Rowe, SM; ACE-2 Blockade &amp; TMPRSS2 Inhibition Mitigate SARS-CoV-2 Severity Following Cigarette Smoke Exposure in Airway Epithelial Cells In Vitro bioRxiv : the preprint server for biology 2024-06-24 [PMID: 38979208] (Immunohistochemistry, Human) Immunohistochemistry Human
Jennifer Hua, Elvira Garcia de Paco, Nathalie Linck, Tangui Maurice, Catherine Desrumaux, Bénédicte Manoury et al. Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer’s disease Cellular and Molecular Life Sciences 2023-05-05 [PMID: 37145189]
István Fodor, Tamar Schwarz, Bence Kiss, Antal Tapodi, János Schmidt, Alex R. O. Cousins et al. Studies on a widely-recognized snail model species (Lymnaea stagnalis) provide further evidence that vertebrate steroids do not have a hormonal role in the reproduction of mollusks Front Endocrinol (Lausanne) 2022-09-08 [PMID: 36157463]
GAE Pickering, F Felix-Ilem, MJ Clark, K Kocsy, J Simpson, I Bellantuon, A Gartland, JM Wilkinson, K Hatzikotou, E Kiss-Toth The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification International Journal of Molecular Sciences, 2022-08-16;23(16):. 2022-08-16 [PMID: 36012474] (Flow Cytometry, Human) Flow Cytometry Human
J Li, MD Chordia, Y Zhang, H Zong, D Pan, Z Zuo Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in mice Theranostics, 2022-03-21;12(7):3024-3044. 2022-03-21 [PMID: 35547761] (IHC, Mouse) IHC Mouse
K Chakrabart, KN Prashanthi, R Shetty, S Argulwar, N Jeyabalan, A Ghosh Generation of iPSC line (GLNNFi001-A) from peripheral blood mononuclear cells of a patient with macular corneal dystrophy Stem Cell Research, 2022-04-22;62(0):102789. 2022-04-22 [PMID: 35472829] (ICC, Human) ICC Human
G Luo, X Wang, Y Cui, Y Cao, Z Zhao, J Zhang Metabolic reprogramming mediates hippocampal microglial M1 polarization in response to surgical trauma causing perioperative neurocognitive disorders Journal of Neuroinflammation, 2021-11-13;18(1):267. 2021-11-13 [PMID: 34774071] (IHC, Mouse) IHC Mouse
D Halperin, A Stavsky, R Kadir, M Drabkin, O Wormser, Y Yogev, V Dolgin, R Proskorovs, Y Perez, H Nudelman, O Stoler, B Rotblat, T Lifschytz, A Lotan, G Meiri, D Gitler, OS Birk CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice Nature Communications, 2021-10-26;12(1):6187. 2021-10-26 [PMID: 34702855] (ICC, Mouse, Transgenic Mouse) ICC Mouse, Transgenic Mouse
T Kang, J Ye, P Qin, H Li, Z Yao, Y Liu, Y Ling, Y Zhang, T Yu, H Cao, Y Li, J Wang, F Fang Knockdown of Ptprn-2 delays the onset of puberty in female rats Theriogenology, 2021-09-28;176(0):137-148. 2021-09-28 [PMID: 34607132] (IHC, Rat) IHC Rat
N Morita, M Hoshi, T Hara, S Ninomiya, T Enoki, M Yoneda, H Tsurumi, K Saito Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity Oncology Letters, 2021-09-17;22(5):790. 2021-09-17 [PMID: 34584567] (ICC, Human) ICC Human
Show All 40 Publications.

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1