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Goat anti-Rat IgG Secondary Antibody [Allophycocyanin]

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bEnd.3 mouse endothelioma cell line was stained with RatAnti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994,filled histogram) or isotype control antibody (Catalog # MAB006),open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113) at 10 μL/106 cells.
bEnd.3 mouse endothelioma cell line was stained with RatAnti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994,filled histogram) or isotype control antibody (Catalog # MAB006),open histogram), followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Allophycocyanin

Order Details

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Goat anti-Rat IgG Secondary Antibody [Allophycocyanin] Summary

Immunogen
Rat IgG heavy and light chains
Specificity
Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
F0113 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rat IgG Secondary Antibody [Allophycocyanin]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (F0113)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(3)
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Mouse
(3)
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Product General Protocols

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FAQs for IgG Antibody (F0113) (0)

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Bioinformatics

Gene Symbol IGHG1