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Human IgD ELISA Kit (Colorimetric)

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ELISA: Human IgD ELISA Kit (Colorimetric) [NBP2-60058]
Human IgD ELISA Kit (Colorimetric) [NBP2-60058] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
0.024 ng/mL - 100 ng/mL
Sensitivity
0.024 ng/mL

Order Details

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Human IgD ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human IgD. No significant cross-reactivity or interference between human IgD and analogues was observed.
Standard Curve Range
0.024 ng/mL - 100 ng/mL
Sensitivity
0.024 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGHD

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate (100 x concentrate)
  4. HRP-conjugate Diluent
  5. Instruction manual
  6. Sample Diluent
  7. Standard (Freeze dried)
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (25 x concentrate)

Alternate Names for Human IgD ELISA Kit (Colorimetric)

  • IgD
  • Igh-5
  • Ighd
  • Immunoglobulin D
  • Immunoglobulin Delta

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgD present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IgD is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol IGHD