IFN-alpha WA/IFNA16 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

IFNA16, also known as Interferon alpha-16, is a 189 amino acid protein that is 22 kDa, belongs to the alpha/beta interferon family, produced by macrophages has antiviral activities, and stimulates the production of protein kinase and oligoadenylate synthetase. Current research is being performed on several diseases and disorders including interferon, dengue hemorrhagic fever, immunodeficiency, hemorrhagic fever, autoimmune thyroiditis, measles, influenza, melanoma, tuberculosis, thyroiditis, hepatitis c, hepatitis, leukemia, and papillomatosis. This protein has shown to have interactions with IFNAR1, IFNAR2, IFNGR1, and IFNGR2 in pathways such as cytokine-cytokine receptor interaction, regulation of autophagy, toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway, cytosolic DNA-sensing pathway, JAK-STAT pathway, all-trans-retinoic acid mediated apoptosis, and hemostasis pathway.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-08143

• IFN-alpha WA/IFNA16 Antibodies
• IFN-alpha WA/IFNA16 Lysate
• IFN-alpha WA/IFNA16 Proteins and Enzymes