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Recombinant Human IFN-alpha H2 (alpha 14) Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-alpha H2 (alpha 14) Protein Summary

Details of Functionality

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.
Source
E. coli-derived human IFN-alpha H2 protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
IFNA14
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
11145-1 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha H2 (alpha 14) Protein

  • IFNA14
  • IFNalpha H2
  • IFN-alpha H2
  • IFN-alpha-14
  • IFN-alphaH
  • interferon alpha-14
  • Interferon alpha-H
  • Interferon lambda-2-H
  • interferon, alpha 14
  • LeIF H
  • LEIF2H
  • MGC125756
  • MGC125757

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

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Publications for IFN-alpha H2/IFNA14 (11145-1)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol IFNA14
Uniprot