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ID1 Antibody (CL11981)

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Staining of human liver shows weak nuclear positivity in hepatocytes.
Staining of human liver shows weak nuclear positivity in hepatocytes.
Staining of human glioblastoma multiforme shows strong positivity in tumor cells.
Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Staining of PC-3 cells using the Anti-ID1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.
Staining of human colon shows strong positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CL11981
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ID1 Antibody (CL11981) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of P41134. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ID1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for ID1 Antibody (CL11981)

  • BHLHB24
  • bHLHb24DNA-binding protein inhibitor ID-1
  • Class B basic helix-loop-helix protein 24
  • dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
  • dJ857M17.1.2
  • ID
  • ID1
  • inhibitor of differentiation 1
  • Inhibitor of DNA binding 1
  • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Background

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ID1