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ID1 Antibody (1F7)

Images

 
Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Analysis of ID1 expression in transfected 293T cell line by ID1 monoclonal antibody (M02), clone 1F7.Lane 1: ID1 transfected lysate(16.1 KDa).Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: ID1 Antibody (1F7) [H00003397-M02] - Analysis of monoclonal antibody to ID1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Western Blot analysis of ID1 expression in Y-79 ( Cat # L042V1 ).
Western Blot: ID1 Antibody (1F7) [H00003397-M02] - Analysis of ID1 over-expressed 293 cell line, cotransfected with ID1 Validated Chimera RNAi ( Cat # H00003397-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot ...read more
ELISA: ID1 Antibody (1F7) [H00003397-M02] - Detection limit for recombinant GST tagged ID1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
1F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ID1 Antibody (1F7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ID1 (AAH00613.1, 1 a.a. ~ 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKVASGSTATAAAGPSCALKAGKTASGAGEVVRCLSEQSVAISRCAGGAGARLPALLDEQQVNVLLYDMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQLELNSESEVGTPGGRGLPVRAPLSTLNGEISALTAEAACVPADDRILCR
Localization
Nuclear
Specificity
ID1 - inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ID1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ID1 Antibody (1F7)

  • BHLHB24
  • bHLHb24DNA-binding protein inhibitor ID-1
  • Class B basic helix-loop-helix protein 24
  • dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
  • dJ857M17.1.2
  • ID
  • ID1
  • inhibitor of differentiation 1
  • Inhibitor of DNA binding 1
  • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Background

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ID1 Antibody (H00003397-M02) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ID1 Antibody (H00003397-M02) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ID1 Products

Array H00003397-M02

Research Areas for ID1 Antibody (H00003397-M02)

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Blogs on ID1

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Bioinformatics

Gene Symbol ID1
Entrez
OMIM
Uniprot