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Human IBSP/Sialoprotein II ELISA Kit (Colorimetric)

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ELISA: Human IBSP/Sialoprotein II ELISA Kit (Colorimetric) [NBP2-70020] - Samples were spiked with high concentrations of Human IBSP/Sialoprotein II and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human IBSP/Sialoprotein II ELISA Kit (Colorimetric) [NBP2-70020] - Samples were spiked with high concentrations of Human IBSP/Sialoprotein II and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human IBSP/Sialoprotein II ELISA Kit (Colorimetric) [NBP2-70020] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

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Human IBSP/Sialoprotein II ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human BSP in samples. No significant cross-reactivity or interference between Human BSP and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.45%
Intra-Assay
CV% < 4.62%
Spike Recovery
87-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IBSP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human IBSP/Sialoprotein II ELISA Kit (Colorimetric)

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

The protein encoded by the Sialoprotein II gene is a major structural protein of the bone matrix. It constitutes approximately 12% ofthe noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophicchondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is thetrophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cellattachment through an RGD sequence that recognizes the vitronectin receptor. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human
IBSP/Sialoprotein II ...
NBP2-70020
Human IBSP/Sialoprotein II ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Product PDFs

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  Human IBSP/Sialoprotein II ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol IBSP