Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary
Background |
The Quantikine Human BAFF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF showed linear curves that were parallel to the standard curves obtained using the Quant...ikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF. |
Specificity |
Natural and recombinant human BAFF |
Source |
N/A |
Inter-Assay |
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Intra-Assay |
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Spike Recovery |
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Sample Volume |
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Applications/Dilutions
Application Notes |
Interference observed with 1 or more available related molecules. |
Publications |
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Packaging, Storage & Formulations
Storage |
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Background
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
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