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Rat HSP90 ELISA Kit (Colorimetric)

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ELISA: Rat HSP90 ELISA Kit (Colorimetric) [NBP2-76450] - Samples were spiked with high concentrations of Rat HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat HSP90 ELISA Kit (Colorimetric) [NBP2-76450] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
125.00 - 8000 pg/mL
Sensitivity
75.00 pg/mL

Order Details

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Rat HSP90 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat HSP-90 in samples. No significant cross-reactivity or interference between Rat HSP-90 and analogues was observed.
Standard Curve Range
125.00 - 8000 pg/mL
Sensitivity
75.00 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.47%
Intra-Assay
CV% < 5.5%
Spike Recovery
86-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HSP90AA1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat HSP90 ELISA Kit (Colorimetric)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • Hsp89
  • HSP90
  • HSP90AHSP86
  • HSP90N
  • HSPC1HSP 86
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

Hsp90 has at least 2 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HSP90AA1