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Recombinant Human HSP27 Protein

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SDS-Page: Recombinant Human HSP27 Protein [NBC1-18364] - HSP27, 22.7kDa (205aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

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Recombinant Human HSP27 Protein Summary

Description
An un-tagged recombinant protein corresponding to amino acids 1 to 205 of Human HSP27

Source: E.coli

Amino Acid Sequence: MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HSPB1
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBC1-18364 in the following application:

Publications
Read Publication using NBC1-18364.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM HEPES buffer (pH 7.5), 100 mM KCl, 1 mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human HSP27 Protein

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for HSP27 Recombinant Protein (NBC1-18364)(1)

Review for HSP27 Recombinant Protein (NBC1-18364) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBC1-18364:
Filter by Applications
In vivo study
(1)
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  5
reviewed by:
Verified Customer
In vivo study 10/11/2023
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Summary

ApplicationIn vivo study
Lot157101

FAQs for HSP27 Recombinant Protein (NBC1-18364) (0)

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Verified Customer
10/11/2023
Application: In vivo study
Species:

Bioinformatics

Gene Symbol HSPB1