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HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

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Western Blot: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Western Blot: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human prostate tissue.
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HeLa cells.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human Kidney tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human liver tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human Ovary tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70947AF350
Alexa Fluor 405
NBP2-70947AF405
Alexa Fluor 488
NBP2-70947AF488
Alexa Fluor 532
NBP2-70947AF532
Alexa Fluor 594
NBP2-70947AF594
Alexa Fluor 647
NBP2-70947AF647
Alexa Fluor 700
NBP2-70947AF700
Alexa Fluor 750
NBP2-70947AF750
Allophycocyanin
NBP2-70947APC
Biotin
NBP2-70947B
DyLight 350
NBP2-70947UV
DyLight 405
NBP2-70947V
DyLight 488
NBP2-70947G
DyLight 550
NBP2-70947R
DyLight 594
NBP2-70947DL594
DyLight 650
NBP2-70947C
DyLight 680
NBP2-70947FR
DyLight 755
NBP2-70947IR
FITC
NBP2-70947F
HRP
NBP2-70947H
Janelia Fluor 549
NBP2-70947JF549
Janelia Fluor 646
NBP2-70947JF646
PE
NBP2-70947PE
PerCP
NBP2-70947PCP
Unconjugated
NBP2-46005
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46005
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HSD17B4 Antibody (OTI4C4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD17B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:4000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

  • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
  • 7-alpha
  • D-bifunctional protein
  • EDH17B4
  • hydroxysteroid (17-beta) dehydrogenase 4
  • MPF-2
  • peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
  • peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
  • peroxisomal
  • short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSD17B4